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KMID : 0620919970290020111
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Experimental & Molecular Medicine
1997 Volume.29 No. 2 p.111 ~ p.116
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Lactoferrin as a gene delivery vehicle to hepatocytes
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Jeong Bin Yim/Sang Taek Oh
Jeong Keun Rih/Heung Sun Kwon/Deog Su Hwang/Sun Young Kim/Jeong Bin Yim
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Abstract
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Using lactoferrin as the specific ligand, we developed a simplified method for preparation of molecular conjugate for gene delivery. Replacement of column chromatography and dialysis by one step centrifugal filtration (Centricon, cut off size : 30,000), resulted in the rapid purification of bovine lactoferrin/polylysine (bLf/pL) and human lactoferrin/polylysine (hLf/pL) conjugates and easy separation of unconjugated polylysine. The Lf/pL conjugates prepared by this method efficiently transferred the reporter genes, CAT and LacZ gene, to HeLa and hepatic cells. The bLf/pL and hLf/pL conjugates could transfer the reporter genes to various hepatocytes including primary mouse hepatocyte, Hepa 1-6, SK-Hep1 and Chang liver, but not to NIH 3T3 mouse fibroblast cells, indicating that the Lf/pL conjugates conferred hepatocyte-specific gene transfer. The bLf/pL and hLf/pL conjugates prepared in the present study exhibited higher transfection efficiencies for mouse and human hepatocytes than the commercially available transferrin/polylysine (Tf/pL) conjugate.
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KEYWORD
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lactoferrin, poly-L-lysine, gene delivery,
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